TECHNICAL DESCRIPTION

This invention describes a new process of non-covalent attachment of a protein (antibody, enzyme), DNA or specific fragment on a solid substrate: slides or beads (porous or non porous). The process can be extended to any kind of ligand: antigen, polysaccharide, specific ligand (drug, hormone, inhibitor). The ligand density and reproducibility is easily controlled. Due to the reversible character of the non-covalent binding, the process permits the release of immobilized molecules under soft conditions, allowing complete regeneration of the initial surface that can be used for new experiments.

INDUSTRIAL APPLICATION

Analytical applications: in sensors (slides or microarrays) using optical (surface plasmon resonance, ellipsometry) or spectroscopic (fluorimetry) detection, the immobilization of molecules bearing molecular recognition sites allows the detection and/or determination of specific antigens or antibodies, sDNA fragments, carbohydrates, proteins in proteomics or enzymes. The main interest of the process is the possibility of sensor regeneration.

Extractive applications: using beads, monoliths or fibers, the process can be extended to prepare selective or specific extractants of the compounds mentioned above. Applications to proteomics may be possible.

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