Find the best CNRS technologies to boost your innovative project.
06.11.2018
Materials – Coating 07293-01
06.11.2018
Materials – Coating 10581-01
06.11.2018
Chemistry / Environment 08758-01
06.11.2018
11127-01
06.11.2018
Environment and Energy 11107-01
19.10.2018
Medical Diagnostic 08504-01
We can guide you through the whole technology transfer process.
Thanks to our expertise, our network and our know-how of the innovation ecosystem, we support you throughout your project.
05287-01
Not patented
Biological material
Marc BORSOTTO
Catherine HEURTEAUX
MTA
Institut de Pharmacologie Moléculaire et Cellulaire, a CNRS and University de Nice Sofia Antipolis (UMR 6097), in Valbonne, France.
TREK-1 potassium channels are involved in a number of physiopathological processes such as neuroprotection, pain and depression. Molecules able to open or to block these channels can be clinically important. Having a cell model for screening such molecules is of particular interest.
The inventors have developed the first available cell line that constitutively expresses the TREK-1 channel.
This cell line has been fully characterized with regards to the modulation properties of the TREK-1 channel that is expressed. The results show that all its properties are retained: it is opened by stretch, pH, polyunsaturated fatty acids and by the neuroprotective molecule, riluzole and it is blocked by spadin or fluoxetine. In addition, the inventors have also demonstrated that the h-TREK-1/HEK cell line is protected against ischemia by using the oxygen-glucose deprivation model.
A) Typical pictures of cells observed in transmission and in fluorescence at round three of cell culture. Functional channel activity was evaluated by current activation with 10 µM AA.
B) Typical pictures of cells observed in transmission and in fluorescence at round fourteenth of cell culture. Functional channel activity was evaluated by current activation with 10 µM AA.
C) Real time q-PCR. Levels of TREK-1 or sortilin expression were normalized with the cyclophillin D expression.
High Throughput Screening on the TREK-1 target for drug discovery.
A human TREK-1/HEK cell line: a highly efficient screening tool for drug development in neurological diseases.
Moha ou Maati H et al. PLoS One. 2011;6(10):e25602.
For further information, please contact us (Ref 05287-01)
25.11.2015
Health / Therapeutic 01310-01, 01310-02, 04666-01
16.09.2014
Health / Therapeutic 01741-01
20.02.2014
Health / Therapeutic, Medical Diagnostic 86609-04
06.11.2018
Materials – Coating 07293-01
06.11.2018
Materials – Coating 10581-01
06.11.2018
Chemistry / Environment 08758-01
06.11.2018
11127-01
06.11.2018
Environment and Energy 11107-01
19.10.2018
Medical Diagnostic 08504-01