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High throughput label-free real-time and in-situ immunoassay by light reflectivity imaging



Statut des brevets

French priority patent application n° FR1256911 filed on July 17, 2012 and entitled “Method and Device for measuring a medium of interest”


Frédéric KANOUFI
Jean-Paul ROGER
Fabien AMIOT

Statut commercial

Exclusive or non-exclusive license



Among label-free systems able to analyze biochemical interactions, the most used is based on surface plasmon resonance SPR. It requires the use of optical prisms and the technique is difficult to scale-down to the micrometer (to microsystems or microfluidic cells) or to scale-up to large dimension immunoassay platforms (>3×3 cm2). The large dimension immunoassay is often used in immunological studies and particularly in the VIH diagnosis. It is based on the use of large scale immunoblots (western blots) : a separation of a mixture of proteins, followed by their transfer on an analytical platform for their selective recognition. The proposed invention requires very simple processes and set-up.


This invention provides a simple and quantitative label-free methodology for the real-time and in-situ kinetic analysis of biochemical interactions devoted to immunoassays performed on reflective surfaces. This invention relates to special arrangements of optical methodology and the controlled transfer of molecules of biological pertinence on a substrate for the design of label-free, real-time analysis of biochemical reaction. It is based on observation through optical microscope of an illuminated surface and the simplest recording of images or movies of these surfaces. The change in refractive index associated with molecule adsorption at a reflective surface is characterized by an attenuation of the reflected light intensity. The real-time in-situ recording (image captures or movies) of the intensity of reflected light from a reflecting surface then allows quantitative kinetic analysis of a surface biochemical reaction. The detection is performed under optimized conditions according to the optical properties of the substrate.
It may be used from miniaturized systems operated in microfluidic platforms to large scale (>cmxcm).


This innovation particularly addresses the analysis of biochemical reactions for different immunoassay formats owing to the use of in situ optical microscopy observation. This innovation could be used for multiple applications as high throughput screening, diagnosis (point of care or high throughput diagnosis), etc.

For further information, please contact us (Ref 04958-01)

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